ABSTRACT
Translational medicine means that to establish a bridge between fundamental research and clinical medicine through bidirectional translation path in order to promote the translation and application from fundamental research to clinical medicine.The purpose of traditional Chinese medicine (TCM) translational model is to collate and build one multidimensional TCM translational model,rationality carry out the research on TCM translation.Three paths have been confirmed,where are from literature research to clinical verification,from clinical application to clinical verification,and from experiment to clinical verification.In this research,methodology of one path mcntioned above could be established,which is the key technology of translational medicine.Take constipation,insomnia,and post-flu cough as target diseases to investigate the herbal compounds which could be qualified for clinical application based on preliminary work.This research not only provided clinical data to TCM translation,but also established one methodology of TCM translation path--"from experiment to clinical verification".
ABSTRACT
This study was aimed to explore the effects of Shengmai(SM) injection on blood pressure (BP),heart rate (HR),electrocardiogram (ECG),and serum C-reactive protein (CRP) and NO levelin Beagle dogs with myocardial ischemia (MI).A total of 30 Beagle dogs were divided into 5 groups,which were the blank control group,model control group,SM injection low-dose group (0.80 mL· kg-1),SM injection high-dose group (3.20 mL· kg-1) and isosorbidemononitrate group (0.07 mL· kg-1).The MI model was established by the subcutaneous injection of isoprenaline hydrochloride,and the drug treatments were given at the same time.At 1 h,6 h,12 h,24 h after administration,changes of BP,HR and ECG were recorded;serum CRP and NO content were detected.The results showed that compared with the blank control group,the mean arterial pressure (MBP) was significantly decreased (P＜0.01),HR was significantly increased (P＜0.01,or P＜0.05),the ECG△ST value was significantly increased (P＜0.01) in the model group.SM injection could improve symptoms mentioned above,but the effectswereslow.SM injection had a significant antagonistic effect on the decreasing of CRP content and the increasing of NO content,compared with the model group.The difference was statistically significant (P＜0.01,or P＜0.05).It was concluded that SM injection could improve the physiological indices of Beagle dogs with MI,which might be related to the regulation of CRP and the inhibition of NO secretion.
ABSTRACT
This study was aimed to screen out a safe and effective traditional Chinese medicine (TCM) compound prescription in insomnia treatment, through the method of uniform design and comprehensive scoring method. It provided candidate drugs to further research and development of TCM new drug. U7(76) uniform design was used to divide groups. The test was given on sleep time prolonging effect of mice injected with pentobarbital sodium. The subliminal dosage of pentobarbital hypnosis was tested on mice injected with pentobarbital sodium. The acute toxicity (LD50) was tested on mice. Comprehensive scoring method was applied to evaluate the effect to determine the optimum dose. The results showed the optimized prescription was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The optimum ratio was 1:3. It was concluded that the uniform design was applied in the experimental design with 3 factors and 6 levels. Comprehensive scoring method was used to evaluate the combined effect. The optimized prescription that put benefit and risk into consideration was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The best ratio was 1:3, which can be further developed.